proteomic analysis Search Results


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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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PTM Biolabs tandem mass tagging proteomics
S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). <t> Proteome analysis </t> was carried out by TopLab.
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S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP).  Proteome analysis  was carried out by TopLab.

Journal: Plant Physiology

Article Title: Regulation of Plant Glycine Decarboxylase by S -Nitrosylation and Glutathionylation 1 [W] [OA]

doi: 10.1104/pp.109.152579

Figure Lengend Snippet: S-Nitrosylated proteins from Arabidopsis mitochondria Mitochondria extracts treated with GSNO or GSH were subjected to the biotin-switch method and analyzed by nanoLC/MS/MS after tryptic digestion. The Mascot search engine was used to parse MS data to identify proteins from primary sequence databases. The best-matching peptide identifying the protein is given. If there were additional peptides found, the number of the peptides is given. The asterisks indicate proteins not listed in the mitochondrial proteome ( Millar et al., 2001 ) but with mitochondrial targeting signal (TargetP). Proteome analysis was carried out by TopLab.

Article Snippet: Proteome analysis was carried out in cooperation with the Helmholtz Zentrum Munich core facility.

Techniques: Sequencing